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We used this structural template, together with the X-ray structure of the ER ligand binding domain, to elaborate an additional hydrogen bonding site on a benzamide system that elevated receptor binding further.
In general, no argument satisfying this template together with a given evidence will be transmissive of first-time justification based on that evidence.
First, the amyQ gene was generated by PCR using pKTH10 (Palva 1982) as template together with ON29 and ON42, the amplicon was treated with Bam HI and Aat II and ligated into pHT01 (Nguyen et al. 2007) cut with the same enzymes resulting in pNDH88.
To test for recombination, we performed PCR using either the total DNA or an artificial amplicon mixture as template, together with primers designed to amplify a bi-species PCR recombination product (Figs. 4 and S2).
The packing efficiency of the model and of its template together with their comparison with the expected value.
The packing environment of residues in the model and the template together with their comparison with the expected value.
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For this purpose, the constructs pMA236, pHP236 and pHP236m [22] were used as templates together with the Gal4 forward primer with extending HindIII and NotI sites ACGCACGCaagcttgcggccgcccaccATGAAGCTACTGTCTTCTATC and the Gal4 reverse primer with extending SalI site ATAGCTGCGTGCGTGCGTgtcgacttactctttttttgggtttgg.
Co-amplification of Xist templates together with the Oct4 templates described above was achieved following the same strategy.
Concordance between genotypes generated from MDA product and gDNA templates, together with the genotype and sample failure rates of each template type were measured.
PCR reaction solution was prepared with 5 μL of the DNA templates together with the PCR mixture to create a total volume of 25 μL.
A dozen times, we tried fitting shapes into the template together.
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