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A fragment of similar size was amplified using aliquots of the cDNA library phage stock as template, to verify the presence of boophilin cDNA in the library.
Each model was compared to its template to verify that the modeling step had not significantly altered backbone and side chain conformations.
The ROI was then displayed on the MRI template to verify its proper location in the peri-aqueducal grey matter of the brainstem.
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Furthermore, IHNV, VHSV, IPNV, KHV, NDV, AIV (H5, H7, H9), CO cell, EPC cell, FHM cell, eggs of sturgeon and salmon, tissues (muscle, pancreas, gill, spleen, fin, brain, kidney) of ornamental carps, common carps, goldfishes, and grass goldfish were used as control templates to verify the specificity of the method.
A nanomicroarray for each target gene was designed, printed in-house, and tested separately by using the PCR products as templates to verify the ability of individual capture and intermediate oligonucleotides to detect a specific target gene.
All assays included no-RT and no-template controls to verify non-specific amplification.
Controls without reverse transcriptase and without template were included to verify that fluorescence was not overestimated by residual genomic DNA amplification or from primer dimer formation.
PCR using genomic DNA as template was performed to verify the integration of MdSPDS1 in the regenerated plants.
Negative controls of cDNA synthesis reactions were conducted in the absence of reverse transcriptase and used as a template in PCR to verify the absence of genomic DNA contamination for each sample.
Each assay included, in duplicate, a no-template control (water) to verify the absence of contamination.
The stocks were used as templates for PCR to verify the positive clones from library screening and to configure the extent of overlap with the existing physical map.
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