A standard curve using the TSR8 template was used to validate quantitative results.
PCR was conducted with (i) different cycle numbers (25, 30 and 32) and (ii) different template cDNA concentrations to validate the linearity of the measured expression values.
For that purpose several auxiliary artifact templates were implemented to validate and connect all the filters following a pipeline of streams that compose a filter graph artifact template.
Then, DNA from 1212, B73, Mo17, and Zheng58 were used as the template for PCR amplification to validate the accuracy of the indel primer design.
The purpose of this study was to develop a 3-dimensional templating technique for the humeral stem and to validate this templating in cadaveric specimens.
For both dye terminator sequencing and SNaPshot assays to validate SNPs, template preparation was carried out in 10 μl in each well consisting of 3.3 μl ddH2O, 1.0 μl 10x dNTPs (2 mM), 2.0 μl 5x colorless GoTaq Flexi buffer, 0.8 μl 25 mM MgCl2, 0.4 μl F and R primers each, 0.1 μl GoTaq Flexi (5 units per μl Promega, Madison, WI), and 2 μl genomic DNA (10 ng/μl).
The graphical user interface (GUI) application Sequin, also available from NCBI, can be used to generate the template file required by tbl2asn and to validate the output of tbl2asn.
In particular, the questionnaire was designed to validate the base IHSD Template that had been derived from the literature, and to obtain quantitative estimates of certain parameters that were not able to be found elsewhere.
Interviews were de-identified prior to analysis, and incorporated along with feedback from the ISC meetings and data from the literature to validate and further refine the template.
