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In the presence of PNK and ligase, the 5′-OH end of a linear Padlock DNA strand could be phosphorylated by PNK and then be converted by ligase into a circular template to trigger subsequent exponential HRCA reaction, producing amounts of double-stranded DNA (dsDNA) fragments, accompanied by the significant fluorescence increase of dsDNA-intercalating dye SYBR Green I (SG I).
The most widely used methods rely on double-strand breaks (DSBs) in the donor template to trigger HR, but these can be time consuming and labor intensive (Gao et al. 2008; Huang et al. 2009).
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Instead, they have a quitting time that is the result of two factors: the need to conserve energy in order to fly back to their own nest (often over 5 km distant for bees living in the wild; Seeley 1985) and the cost of continued attempts, which is higher than for nestmates because the robber is assumed to differ more from the guard's template and to trigger greater aggression.
Showcasing the efficiency and sequence specificity of native chemical PNA ligation, attomolar template proofed sufficient to trigger signal while a 1000-fold higher load of single mismatched template failed to induce appreciable signal.
Note that the "abort" command is used instead of "stop", since "abort" does not generate notification (onEnd) to trigger the link "changingPhotos" inherited from the template of Listing 3. The template we present in this section is similar to the Button-Text-Image template of Sect. 3.
Hence a template of gradated threats should be available instead, with the commission being able to trigger some of them without consent from member states, but possibly in co-operation with the European parliament.
Ashley Banjo knows how to trigger audience response.
That seems to trigger the desired reprogramming.
Time to Trigger.
That started to trigger our attention".
Set conditions to trigger the rule.
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