96-well plates were used for each run, including 6 standards for each viral type and HMBS, involving 10-fold plasmid dilutions 10-100 copy dynamic detection range) and a no template control to rule out DNA contamination.
Each run was performed in 96-well plates, including 6 standards for each viral type and HMBS, involving 10-fold plasmid dilutions (10 10 copy dynamic detection range) and a no template control to rule out DNA contamination.
Seven parallel negative controls without a DNA template were assayed to rule out the possibility of contamination of the highly sensitive MAP assays.
At all steps, a series of controls without DNA template was used to rule out contamination.
In addition, a nontemplate control containing all reaction mixtures except the template was included to rule out contamination of the reagents.
For each of the target genes, control reactions without template were performed in order to rule out contamination.
Developers also have to write a monitoring rule template to know from an agenda, if there is a meeting or not and if a meeting will be held in less than 30 min, after a previous meeting.
Even informal "blue-sky" sessions were stifled by reminders to use the company's standard PowerPoint template and adhere to rules about maximum words per slide, which reinforced the feeling that people needed to stay within certain bounds.
In addition, because we used exon-specific primers and DNA as a template, we cannot rule out the presence of some mutations not detectable by this PCR approach, for example, heterozygous deletions spanning one or several exons.
