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Gradient end-point PCRs were used to optimize the annealing temperatures and RT-qPCR was performed on serial dilutions of the template to optimise the reactions for each primer pair.
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The hypergeometric probabilities for the ADP comparisons were significant only at an input of 50 ng total RNA; this may reflect suboptimal performance of the reverse transcription and a need to optimise the primer:template ratio.
Although it is necessary to optimise the dsDNA Fragmentase reactions for a given template, this proved to be an efficient method for fragmenting the long-range PCR products of a large number of individuals, and produced consistent results.
Many brands are still lagging behind in mobile optimisation, as desktop is far easier to optimise.
This assessment thus allows kiwifruit industry in New Zealand to optimise survey design to the desired information, and provides a template for other industries to do likewise.
Rather we should try to optimise several.
That shared desire to optimise potential still unites us.
Such user data is then used to optimise city layouts.
Want someone to optimise your CV? Five dollars.
These companies compete for business by promising to "optimise" conditions.
This uses hydraulics and electronics to optimise valve settings.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com