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DTK271 genomic DNA was used as a PCR template to isolate the ade2 -min3 allele using primers 14193006 and 14193007.
The eight TC sequences (Table 1) corresponding to new wheat PDI-like genes were used as template to isolate by RACE the corresponding 5' and 3' extensions, subsequently validated by sequence analysis.
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For each subject, 3D T1 sequence was normalized to the SPM8 template, then segmented to isolate gray matter and white matter partitions, and modulated for deformations.
The genomic DNA isolated from P. glauca was used as the template in PCR to isolate the complete coding sequences of PgD5 and endopiceasin.
Pinotage were used as templates in PCR strategies to isolate possible Vitis defensin sequences.
A microfluidic reverse transcription-PCR chip was also utilized to isolate mRNA templates by using beads coated with poly-T nucleotides, as well as to synthesize cDNA from individual cells [ 71].
Genomic DNA from Pseudomonas putida 4G-1 was isolated and used as template to amplify another putative aroA gene (designated as PparoA2).
For instance, first AAO template has to be isolated from Al substrate, and this is achieved by dissolving the Al substrate in a toxic saturated solution of HgCl2.
The N. meningitidis DNA template was isolated and purified from bacterial culture.
One of the early methods for doing site-directed mutagenesis was the Kunkel method [ 11] that uses template DNA isolated from a ung- dut- E. coli strain.
The TaWRKY53 CDS was amplified with primers TaWRKY53exp_fwd1 and TaWRKY53_exp_rvs1 (Table 1) from a cloned full-length cDNA template previously isolated from GR wheat.
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