Sentence examples for template to introduce from inspiring English sources
The suicide plasmid was used as a template to introduce mutations into the mreC gene by inverse PCR.
Synthesized structurally the rigid template having 1,3,5-triazine as core moiety, has been used as template to introduce two appropriately spaced amino groups for substrate binding on the solid surface of silica.
The GFP-tagged III-IV linker construct was used as a template to introduce by site-directed mutagenesis the R1573P and T1606M mutations using overlap extension PCR method, and the PCR products were inserted into the XhoI/HindIII sites of pEGFP-C1 vector.
To test whether Cas9n can induce precise gene targeting via homologous recombination at a gene locus not expressed in hESCs, we designed the experiment to employ Cas9n and a long DNA donor template to introduce a drug resistance gene into a lineage-specific locus.
For construction of double mutants we used pHT315Ab-D136N harboring a point mutation D136N as template to introduce additional point mutations as E129K or T143D.
Ins2 cDNA was then cloned into pCMSGFP and the resulting plasmid was used as a template to introduce mutations associated with human diabetes, using the QuikChange site-directed mutagenesis kit (Stratagene).
We have modified this by using the template pattern to introduce the concrete subclasses to represent the different workload patterns and to implement the workload pattern context as an autonomic element.
Here, Cas9 and sgp53-1 or sgp53-3 were introduced into Arf −/− mouse embryonic fibroblasts (MEFs) in the presence or absence of a corresponding oligonucleotide template designed to introduce a premature nonsense mutation (Fig. 4a).
By comparison, depending on the specific donor, 1 19% of mutant progeny of flies injected with ZFNs and ssODN donor templates designed to introduce stop mutations in a rosy exon reflected HR-mediated repair (Beumer et al. 2013).
Here, we report an intriguing template casting strategy to introduce a mass of atomically dispersed FeN2 moieties onto the surface of N-doped ordered mesoporous carbon for boosting ORR electrocatalysis.
A 7 kb PvuII/ BamHI fragment containing exons 2, 3 and 4 and mutant exon 5 in a pBluescript plasmid vector was used as a template for PCR to introduce PTCs (E77X or K91X) into exon 3 or 4 by site-directed mutagenesis (Stratagene, La Jolla, CA, USA).
