Sentence examples for template to determine from inspiring English sources
Standard PCRs were run with fivefold dilutions of the cDNA template to determine the optimal amount of template and optimal annealing temperature for the experimental and reference reactions, using 500 nM of each primer.
In this study, we used pMIR-Report-human 433/127, pMIR-Report-rat433/127, pMIR-Report-dog433/127 plasmids as the standard template to determine the PCR efficiency, then compared the expression level of miR-433 and miR-127 primary transcripts.
IGF2R was chosen as a template to determine if the same additives, DMSO and betaine used to successfully amplify the fully formed gene fragment [25] could also be employed to aid in building and amplifying it from a pool of overlapping, single-stranded ODNs.
The PCR product from this reaction was then used as the template to determine autosomal linkage.
We subsequently unzipped the DNA template to determine if Gal4DBD was still bound.
Therefore we used the contralateral intact acetabulum and femoral head as mirrored template to determine the estimated HJC.
Specifically, two one-step TaqMan-based multiplex RT-qPCR assays were developed and tested with target templates to determine sensitivity and detection efficiency.
Computed Tomography scans of 204 healthy hips were digitally analysed and manually templated to determine principle dimensions, appropriate stem size and model, as well as cortical distance at the femoral calcar (ideal collar size).
The Georgian teenagers used the Prix Jeunesse Youth Jury judging templates to determine the Top 5 programmes.
These annotations, together with the manually curated A. salmonicida genome, were used as templates to determine the function of genes from all 64 genomes.
These fragments are eluted from the gel and used as PCR templates to determine nucleosomal occupancy of the proximal EGFR promoter as described in the preceding section.
