Sentence examples for template to detect from inspiring English sources

The DNA was used as template to detect the NRPS genes of the endophytes.

Human endogenous retroviral sequences were used as a template to detect homologous endoretroviral sequences in other primates, whenever possible.

Semi-quantitative PCR was performed using the first strand cDNA as a template to detect cystatin C, cathepsin B, and GAPDH mRNAs.

cDNAs from three testis samples at each stage were used as template to detect the expression changes of the miRNAs, and all PCRs were performed in triplicate.

We used negative controls of PCR amplification (i.e. absence of DNA template) to detect contaminations derived from the presence of bovine (because of the use of BSA) and human DNA.

1 μL cDNA was used as RT-PCR template to detect c-Myc and EGFP-Luc mRNA expression.

The genomic DNA of the colonies grown on the plate during each generation were extracted and used as PCR templates to detect the presence of the AaIT gene in Bb-AaIT.

Total RNAs isolated from roots (R), stems (Ste), leaves (Le), bracts (Br), stamens (Stm) and carpels (Ca) were used as templates to detect the expression of CsPI by quantitative real-time PCR.

The genomic DNA of every generation was extracted from the 4-day-old colonies according to a documented method (Raeder and Rroda 1985) and used as PCR templates to detect the AaIT gene presence in the recombinants with SP-AaIT-F and SP-AaIT-R primers (5′-CCCAAGCTTATGCGTGAACTTTCTTCGGT-3′ and 5′-CCCTCGAGTTAGTTGATGATGGTGGTAT-3′, respectively).

The technique utilizes differentially labeled tumor DNA and normal tissue DNA as competing probes and normal metaphases as templates to detect and localize gains and/or losses of genetic material across the entire tumor genome [ 11].

The regular PCR reaction with Mango Taq Polymerase was performed on the above cDNA samples as templates to detect the presence of GPER using specific primers (FP 5′ AGTCGG ATGTGAGGTTCAG 3′ and RP 5′ TCTGTGT GAGGAGTGCAAG 3′) for GPER and Human ribosomal phosphor-protein (36B4) as reference marker [ 76].