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Following this process, one CW of the normal condition is selected as a template to calculate the distance of DTW (DDTW) with other CWs.
All survival replicates were checked for consistency prior to being combined into single survival curves in Microsoft Excel using a macro-based template to calculate Kaplan-Meier survival probabilities every 24 hours.
Target mRNAs were quantified in triplicates for each template to calculate the ∆Cts between the target mRNAs and their degradation intermediates using arithmetic means.
For the modeling of the 3D structure, a BLAST [ 83] was performed to find an approaching structure in PDB database [ 84] in order to use it as a template to calculate a model with MODELLER [ 85].
Because the absence of a P. harei codon usage table, two other closely related firmicute bacteria codon usage tables, Clostridium thermocellum ATCC 27405 based on 3191 CDS's (1,072,649 codons) and Lactobacillus gasseri ATCC 33323 based on 1755 CDSs (558,761 codons) served as template to calculate CAI and the average CAI-values from the two different bacteria were used.
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The ribosomal protein S7 gene was used for normalization of the cDNA templates and to calculate the threshold values (Ct).
A Microsoft Excel template was prepared to calculate viral loads, and E2/E6 ratios (see Additional file 1).
A standard curve was generated, based upon measurement of the reactions performed with a TSR8 (control template), in order to calculate the amount of TS primers with telomeric repeats extended by telomerase in each tumor extract.
Magnetization prepared rapid gradient echo and fluid attenuated inversion recovery images were coregistered to a standard template and segmented to calculate regional brain tissue and WMH volumes normalized for intracranial volume (SPM7, University College London, U.K). (14) (IDL, Research Systems, Boulder, CO; MATLAB, MathWorks, Natick, MA) (12, 13).
Amplicon specificity was determined from the shape of the melting curve and the difference in cycle threshold (Δ Ct) between methylated (MSRE cleavage protected) and unmethylated (MSRE cleavage unprotected) templates was used to calculate cleavage efficiency.
Balance sheet templates are programmed to calculate the sums of your assets, liabilities, and equity, and automatically do the balance calculations.
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