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Some of the crossover sites were characteristic of classical recombination hotspots with a stretch of AU-rich sequence that promotes dissociation and switching of RNA polymerase from one template to another [40], [41].
These were chosen to maximize the possibility of detection of recombinant sequences, which requires clearly differentiated viral populations, while simultaneously maximizing the possibility of contiguous pairing enabling RNA polymerase shifting from one template to another while keeping the same position and reading-frame.
All providers in the division used the process, but some evolved from one template to another over time.
Not surprisingly, during cDNA synthesis in vitro reverse transcriptase can also switch from one template to another and, thereby, generate artificially deleted or chimeric cDNA transcripts [ 96– 96].
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Genomic DNA from Pseudomonas putida 4G-1 was isolated and used as template to amplify another putative aroA gene (designated as PparoA2).
To produce a protein from a gene, the DNA sequence that makes up the gene is used as a template to create another molecule called messenger RNA.
Under these circumstances, when a DSB-bearing chromatid replicates to yield a sister chromatid, the two sister chromatids will be unable to provide a useful repair template to one another.
A 1 µL of amplified PCR products (D1 D5 and GAPDH) from the first step was used as a template to initiate another PCR reaction, which was performed under the same conditions as first step except that different primers were used for the nested PCR (Table 1).
Cut around the card and use this as a template to make another one of the shapes.
Thereafter, this standard is being used as a template to build the structure of another epitope (see methods).
In a gene conversion process a part or the whole sequence of one of the paralogous genes is used as a template to modify the sequence of another.
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