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The structure of the binding domain (L36 scFv) was modeled by comparative modeling [23] using as template the structure of 2GHW.B from the Protein Data Bank (PDB) [24].
We have also modeled yeast PHO36 (UniProt code: Q12442, region: 80 300) using the same procedure described for human ADIPOR1 and as template the structure of archeal (Natronomonas pharaonis) phototaxis protein rhodopsin II (PDB code: 1GU8).
Modelling was carried out by homology at the EsyPred3D Web Server 1.0 [41] using as template the structure of the TRXh1 from Arabidopsis thaliana (AtTRXh1: PDB entry 1XFL) which shares 70% and 64% similarity with PsTRXh1 and PsTRXh2, respectively; the structure of the TRXf from spinach (1FAA) was used for modelling the structure of PsTRXf (77% of sequence identity) [42].
The pdb files for human eEF1A1 and eEF1A2 were generated at Swiss-Model Server using as template the structure of yeast eEF1A (PDB ID: 1F60, chain A).
Because the RNA polymerase subunits RPA2, RPB2 and RPC2 are similar in structure, we used as our common template the structure of RPB2 from S. cerevisiae [ 42, 43].
A theoretical 3D structure of GDH4 from Chlorella sorokiniana was calculated with the homology-modeling program ESyPred3D that creates a PDB-like file, using as the template the structure of bovine GDH3 (PDB # 1HWZ).
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Finally, segmentation was completed by fusing the optimal 3D template and the "structure of interest".
as templates, the structures of either SiCYS-N or SiCYS-C showed similarity as those using Orzyacystatin I as templates (data not shown).
The PDB code is the code of the template structure utilised for the comparative modelling in the PDB database [64].
The goal of this experimental study was to evaluate the effect of the template structure on the comprehensibility of SPL use cases.
We used as a structural template the X-ray structure of opsin (PDB code 3CAP [3CAP.
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