To further reduce polymorphism in the template, the small-insert sequencing libraries used in assembly were generated from the DNA of single female mosquitoes rather than pools of individuals, using whole genome amplification to increase the quantity of available DNA required for the 3- to 5-kb insert libraries.
The lower the abundance of any template, the smaller the probability is that its true abundance will be maintained in the amplified product.
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When the same amount of template was used, the smaller diameter template produced a catalyst with larger surface area and pore volume.
Eight hundred ng of total RNA were used as a template to obtain the small RNA library and we used the SOLiD RNA Barcoding System (Ambion Inc., USA) for library multiplexing.
We refer to the k templates with the smallest distances as "surrogate family members" because they (ideally) share recent ancestry with the study individual.
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This indicates that the PCR based amplification technique yields a cDNA population that resembles with high fidelity the original template population present in the small number of cells used.
These techniques showed a very high level of correlation indicating that the PCR based amplification technique yields a cDNA population that resembles, with high fidelity, the original template population present in the small number of cells used to prepare the cDNA for use with the chip.
High-magnitude, positive values reflect a good match to the template whereas small magnitude values reflect a poor match to the template.
Figure 2a shows a SEM image of the inverse opal structure derived from using large silica spheres (3 μm in diameter) as the template with small polystyrene spheres (200 nm in diameter).
