Sentence examples for template the sequence from inspiring English sources

Human HSF1 (hHSF1) coding sequence (Accession no. NM_005526.2) was amplified by PCR using cDNA from WM793B cells as a template; the sequence recognized by HindIII restriction enzyme was introduced into primers.

Quantitative Real-time PCR experiments were performed in 20-μl reaction mixture containing 5 ng of cDNA template, the sequence-specific oligonucleotide primers (purchased from MWG-Biotech, Gmbh, Eurofins Genomics, Hamburg, Germany) and the Power SYBR Green PCR Master Mix Applied BiosystemsSYBR Green PCR Master Mix Applied Biosystems

Top row: schematic of experiments with DNA templates containing the sequence of F1 target (left) and DNA templates lacking the sequence of F1 target (right).

The adsorption selectivity of synthesized Cu II -IIPs with different anions in the templates follows the sequenCu II -IIPs> Cl− > SO42− > NO3−.

The single strand was used as a template for the sequencing of 532 bp with an automated DNA sequencer (Macrogen Inc).

Five µl of the purified PCR fragment was used as a template in the sequencing reaction.

PCR products were diluted with 20 µl water and 1 µl was used as template for the sequencing reactions.

The sequencing electrophoregram of the control template with the sequencing primer Gre is shown in Figure 4.

After inactivating the enzyme at 80°C for 15 min, we used 0.5 μl as template for the sequencing PCR.

PCR products were diluted with 25 μl water and 1 μl was directly used as template for the sequencing reactions.

The Smith-Waterman algorithm with Gotoh's improvement was used for matching the reads to template sequences in the JAligner software package [ 16, 17].