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To ensure that the eight subjects contributed equally to the final template, the mean activity in the basal ganglia was normalized to a value of 1. Spatial normalization of PET images to raclopride PET template.
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The images were stereotactically normalised into the standard space defined by the Montreal Neurological Institute MNII) template using the mean of the functional volumes, and smoothed with a Gaussian filter of 6 mm full-width at half-maximum.
The images were then stereotactically normalized into the standard space defined by the Montreal Neurological Institute MNII) template using the mean of the functional volumes, and smoothed with a Gaussian filter of 6 mm full-width at half maximum to increase signal-to-noise ratio and facilitate group analysis.
The registration template is the mean face obtained from the training set.
We obtained the customized SPECT template as the mean of all the latter normalized SPECT images.
Each reaction was performed in triplicate for each template using the mean value of the wells for final calculation.
The template represents the mean local waveform of the subjects with normal vision and it is independently scaled in both amplitude and time to fit the individual local responses.
The anatomy of the template brain is meaningful, because each location in the template brain is the mean location of the cortical loci in individual brains that were warped to that location.
All available PET data sets (n = 213) were non-linearly spatially normalised to a population-specific PIB-PET template based on the mean PIB data from the 151 individuals who had MRI data available.
All PCR reactions were performed in triplicate for a technical replicate, including no-template controls, and the mean of the triplicates was used.
All PCR reactions were performed in triplicate for a technical replicate, including no-template controls, and the mean of triplicates was used.
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