Sentence examples for template the expression from inspiring English sources

Using pK6 (Fukuda et al. 2013b) as a template, the expression cassette of the kanMX4 marker (consisting of P TEF1, ORF and terminator) was amplified with oligonucleotide pair o7 and o8, and inserted in place of the P PGK1 -EGFP-T atH1 cassethe at the SacII-XhoI sites of pHY-PGA (Fukuda et al. 2013a), yielding a plasmid designated pHY-kan.

As control for equal amount and quality of template cDNA, the expression levels of the Mlo gene [27] were determined.

Pulsatile patterns were discovered by convolving a template with the expression signals [ 137].

This was done by comparing pathways, using gDNA as a template, with the expression levels of RNA.

As a template control, the expression of a fragment of the 28S rDNA was observed in all samples at essentially the same level.

Having infected a hepatocyte, viral rcDNA is converted to covalently closed circular DNA (cccDNA), which serves as a template for the expression of viral genes and for the formation of the replicative intermediate pregenomic RNA [ 19- 21].

The use of nucleic acid templates for the expression of tumor-derived antigens allows for the expression of the antigens as full-length proteins within dendritic cells.

The AtTUBRTF and AtTUBRTR (primers for Arabidopsis tubulin gene in Table  3) were used to verify equal concentration of templates for the expression analysis.

RNA preparations from cell lines MCF10A, LNCaP, Jurkat, L428, SUDHL6, and OCI-Ly3 were used as probe templates, and the expression of each gene was compared directly with that of the same gene in the Human Universal Reference (HUR) RNA from Stratagene.

cDNAs from three testis samples at each stage were used as template to detect the expression changes of the miRNAs, and all PCRs were performed in triplicate.

cDNAs from three Taihu and three Large White ovary follicles were used as the template to detect the expression changes of the target genes.