Sentence examples for template the double from inspiring English sources

Site-directed mutagenesis was performed following the manufacturer's instructions (QuikChange Site-Directed Mutagenesis Kit) using as template the double mutant AfUGMK344A/K345A gene cloned into pVP55A, while the TcUGM and MtUGM genes were cloned into pVP56K.

The Cas9 and gRNA expression plasmid used in this study can be applied for genome engineering purposes by additionally introducing an editing DNA template to repair the double strand DNA breakage caused by Cas9 in the genome of L. citreum.

To encourage complete cleavage of the template, double digestions were performed for both the MS- and MDRE digestion reactions.

All LEDs with the PSS-ANP template had doubled the light intensity of the LED without the PSS-ANP template at a low injection current between 10 and 40 mA.

The Rad51 filaments formed on unconstrained ds templates progress freely, unwinding the double helix (∼19 vs. 10.5 bp per helical turn) and stretching the DNA by a factor of 1.5 [15].

You don't have to use duct tape; you can use things like gum wrappers, decorated paper, comic strips and other things if you stick them to the template with double sided tape.

Marty himself has been something of a hipster template for a while now: the double denim, the checked shirt, the high-tops.

Subsequently, the double titanium osteotomy template with correct cut-angulation was constructed.

To achieve a good organization of the AAO template, a double anodization process [20] can be used.

In order to increase the amount of possible template, a double round PCR approach was performed.

A negative control was set up by substituting the template with double-distilled H2O.