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Using the amino acid sequence as the starting template, the corresponding DNA sequence was generated by reverse translation and optimized for expression in human cell lines using Genscript's OptimumGene codon algorithm.
To generate prey-tagged expression vectors used in co-immunoprecipitation assay or immunofluorescence, the coding regions of various genes were amplified by PCR using as template the corresponding EST clones obtained from RZPD (Berlin, Germany) and then inserted in the expression vectors using Gateway technology (Invitrogen, Carlsbad, CA).
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These supramolecules can encapsulate guest molecules like the building block [Cp*Fe η-P5)] itself,[Cp*Fe η-P5carborane,[ 6f] C60,[ 6g] or ferrocene.[ 6c] Based on the template effects, the corresponditself- or 80-vertex balls are formed around these templates.
If there are different materials, simply label each copied template with the corresponding material type.
When issuing a TPM command, it replaces the template with the corresponding parameters without allocating new memory in the heap.
In this study, a note is removed from the analysis process by removing its template and the corresponding intensity information.
A transmission scan using computed tomography (CT) or rotating rod sources is performed to obtain an attenuation template of the corresponding hardware component [21 24, 30 32].
By calculating the absolute value of the dot product between each eigenvector from the template and the corresponding eigenvector from the subject's striatum, we obtained a tern of values included in the interval (0, 1).
The sequence identity between the helix 7 of the template and the corresponding helix of the target is only 6%, and the global sequence identity is only 27%.
The initial template and the corresponding sequence alignments of GmRLK18-1 were chosen from the 3D-Jury server (http://BioInfo.PL).
Acyltransferase genes were amplified by PCR using Y. lipolytica W29 genomic DNA as the template and the corresponding primer pairs (Table 2) as described in the previous section.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com