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But if scientists supply no template, the cell cannot precisely repair the break, and the gene usually ends up with a deactivating "scar," where some nucleotides go missing or some are added that don't belong.
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In eukaryotes these protein-DNA interactions occur in the context of a chromatin template within the cell nucleus.
The centrosome is the primary microtubule organizing center of the cells and templates the formation of cilia, thereby operating at a nexus of critical cellular functions.
Extracellular matrix (ECM) acts as an instructing template for the cells contained in tissues.
Either v53 or c53 was used as the model RNA template for the cell-free reactions.
In one of CRISPR's original uses, a template DNA tells the cell's machinery how to repair the double-stranded break, allowing edits as precise as the changing of a single nucleotide.
Sections from the frozen blocks of tissue provided a histological template for characterizing the cell composition of the adjacent non-frozen tissue blocks.
To quantitatively determine the level of mRNA expression of the PRDX6, cDNA templates of the cells were amplified by real-time quantitative PCR.
The Xenopus system is ideal to study the rapid activation process of ATM following addition of DNA templates to the cell-free extract (Costanzo et al, 2004b).
Highly porous apatite-based bioceramic scaffolds have been widely investigated as three-dimensional (3D) templates for cell adhesion, proliferation, and differentiation promoting the bone regeneration.
A least-squares fit of a constant times the cell template was then performed to the observed cell image.
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