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Using the recombinant plasmid pUC57-PCV3 as template, the analytical sensitivity was 23 copies.
When mutant-specific primers for the 15 bp deletion contain multiple mismatches (2 5 bases) with the wild-type template, the analytical selectivity of the assay is >109 (Fig. 2B D, Table S2) and 100,000-fold greater than that observed in PAP-A, which contains only one base mismatch at the 3' end (analytical selectivity ≤104) (Fig. 2A).
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Plasmids containing the wild-type sequence served as the template for the analytical specificity test.
Plasmids containing mutant sequence served as the template for the analytical sensitivity test.
The following is a simple, tried and true questionnaire that forms the analytical template my partner, Greg Hicks, and I use when we're working with Senior Executive Teams.
But the follow-the-money analytical template at the heart of Marxian analysis remains as apt now as it ever was.
The DNA templates were used to evaluate the analytical sensitivity of the Qiaxcel-B assay.
To determine the analytical limit of detection, serially diluted variant templates (1 × 10 1 × 10 copies/ μL) were tested.
The analytical sensitivity of simultaneous detection of the seven premixed bacterial templates was about one order of magnitude higher than that of individual target.
The analytical window of the assay covered at least 5 orders of magnitude of template DNA input with a detection limit in the low picogram range of genomic DNA.
Rochelle is the analytical arm.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com