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Basu et al. (2014) were able to generate a 6-nt pre-translocated transcript (containing a 5′-triphosphate) in crystals of Tth transcription initiation complexes with a downstream-fork promoter template that lacks duplex DNA upstream of the −10 element and is therefore unable to 'scrunch' the t-strand DNA.
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The 36-mer oMMN08-465 was the template strand and the 35-mer oMMN08-466 was the non-template strand that lacks A (complementary to −56T) at its 3'-end.
The negative control was an siRNA template sequence that lacks significant homology to the mouse, human, and rat genome databases.
In the cells that lack a template (G1, early-S) DNA repair relies on the NHEJ which is error-prone due to a possibility of a deletion or rearrangement of some base pairs [ 13- 17].
As a negative control, each plate contained a minimum of four wells that lacked the cDNA template.
Samples that lacked either a template or reverse transcriptase were used as controls.
A model for S-PTGS proposes that transgene-derived aberrant RNAs that lack a poly(A) tail or 5′ capping are used as a template for RDR6 to produce dsRNA, thereby triggering PTGS [ 4].
But that lacked something.
Howard Jacobson on novels that lack redemption.
Those that lack big products in development.
If sequence similarity of the entire serpentine domain (the region from the start of TMH1 to the end of TMH7) and helix 8 is used as the sole criteria for homology modelling template selection then a template may be selected that lacks a particular functionally important structural feature.
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