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Part of the JET template that generates Java classes in the framework source-code from the features found in the F3 models are seen as follows: Figure 4 Hierarchy of the templates which compose FM.
The minimum copy number of a template that generates a detectable product when P* matches the mutant template.
The OMTT's format was developed with a dynamic construction, which utilizes a single flexible template that generates the course by placing assets (text, images, videos, interactive exercises, animations, etc).
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The RNP serves as the template for replication that generates viral genomic RNA in the form of an RNP ready to be packaged in the virion.
Quantitative PCR was performed with cDNA as a template that was generated from reverse transcription with SYBR Green (Sigma) to quantify relative levels of cDNA.
JC developed or executed the tools that generate structures, templates, domains, DNA binding sites, and multiple sequence alignments.
Extensive self-complementary primers or those complementary to the apposing primer were rejected, as were any primer pair that generated non-specific products in a no template control amplification or that generated amplification efficiencies <99%.
In these quantitative analyses, the number of copies of sense and antisense RNA was determined based on the net difference between signal generated from specific cDNA template and that generated from primer-independent cDNA template.
methyltransferase-like primers 5′ TAA TAC GAC TCA CTA TAG GGA GAC AGC TCT GTG GAT CTG GTC A 3′ and 5′ ATT TAG GTG ACA CTA TAG AAG AGT CTC TAA GGT GCG GCT TGT T 3′ were used to make a PCR template that was used to generate a digoxygenin-labelled RNA probe using a Roche RNA labeling kit.
Quantitative real-time PCR (qPCR) was performed on cDNA templates that had been generated with oligo-dT and random hexamer primers from total RNA isolates, using Power SYBR Green PCR Master Mix (Applied Biosystems) and an ABI Prism 7700.
A poly-nucleosomal template was generated, comprising a DNA template that includes twelve 5S rDNA nucleosome-positioning sequences and histone octamers.
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