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On 3 April, the synthetic HA coding sequence was cloned into a DNA template that contained the RNA-dependent RNA polymerase genes, SAM vaccine genetic control elements and a T7 polymerase promoter.
Competitors: 'self' indicates the same DNA template without the biotin label, 'GDF' indicates the non-labeled DNA template that contained the Pol III promoter element obtained from the GDF15 promoter, and 'Mut' indicates the Pol III A-box element was mutated in the DNA template.
To further explore the effects of local sequence context on the kinetic signatures of 5mC and 5caC, we used a synthetic SMRTbell template that contained a modified base in a 5'-CG-3' sequence context, surrounded by two random bases on each side.
Logistic regression models were used to compare the odds of documentation of each process among patient visits having a template that contained the relevant pre-printed items compared to patient visits that did not have a template with relevant pre-printed items, controlling for covariates.
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Semi-quantitative data was obtained from all PCR/ESI-TOF-MS analyses as each well of each assay is seeded with a DNA template that contains the appropriate primer binding sites for the primers in that well.
This study examined the bypass of γ-OH-PdG by Sulfolobus solfataricus Dpo4, the prototypic Y-family DNA polymerase, using templates that contained the adduct in either the 5′-CXG-3′ or the 5′-TXG-3′ sequence context.
New VM instances are created from static templates that contain the basic configuration of the VM to achieve elasticity with regards to capacity.
Recent advances in elucidating the molecular mechanisms of carcinogen-induced mutagenesis indicate that replication of DNA templates that contain replication-blocking adducts is accomplished with error-prone DNA polymerases.
Expression from both AUG codons was reduced, indicating that both translation products were derived from mRNA templates that contain both AUG codons.
In order to seek confirmation of our hypothesis, we devised a crystallisation experiment with an RNA primer/ DNA template substrate that contained an RNA 9mer, i.e., one base shorter of the primer used in the original crystallization experiment.
No-template controls that contained 2 μl of PCR water instead of DNA and positive controls that contained DNA of Brucella melitensis were included in each run to detect any amplicon contamination or amplification failure.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com