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Using a template with a specified shape, diameter, density, and arrangement allows a large number of desired targets to be duplicated once the specific template structure has been formed.
The RCC1 template structure has a pseudo-sevenfold symmetry with a propeller-like shaft made up of seven blades (B1 B7) consisting of four-stranded anti-parallel β-sheet.
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The subjects using this template structure had better results in terms of time spent.
The subjects using this template structure had better results in terms of accuracy; ERQ2: Which of the evaluated template structures requires less time to understand a use case?
Combining these structural analyses with quantitative analyses of sequence similarities between the template structures has allowed us to develop workflows for template selection for each of the seven TMHs and helix 8.
Model quality was checked using ProSA-web [ 99] to ensure the model and template structures have comparable z-scores (an standardized indicator of a structure's total energy compared to that expected by random chance), and by ProCheck [ 100], to ensure bond lengths and angles do not have unusual stereochemical conformations.
Interestingly, a similar 5′ template overhang structure has also been observed in the ternary complex of yeast pol δ (17), suggesting that this structure might also have functional significance.
In comparison, the template-stripped structure has smooth surfaces, similar to those previously demonstrated for Ag and Au.
A model for mutated human NANOS3 was built using the resolved structure of L3MBTL1 (PDB-ID 2RI7) as template since NANOS3 structure has not yet been resolved.
Of all the available templates in PDB presently, this structure has the best resolution (the best resolution of ribosome-SecY complex is only 7.1 Å).
Rhodopsin crystal structure has been used as a template for creating mGluR2 models.
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