Sentence examples for template solutions were from inspiring English sources

The corresponding data of the three template solutions were analyzed by PCA, obtaining 100% recognition.

The organic template solutions were made up to a concentration of 0.10% m/m.

Master mix/primer plus primer probe solutions and template solutions were separately loaded onto 96 well source plates.

The template solution was denatured by the addition of 3.5 µl buffer DLB for 5 minutes at room temperature.

For simultaneous detection of HBV and HCV, 2.5 μl of each template solution was added.

Each template solution was split into two equal parts with half serving as an original template for the measurement of the damaged/relaxed mtDNA fraction and the other half heat-treated (95°C for 6 min on a PCR machine) to quantify total amount of mtDNA [ 31].

Subsequently, 1.0 mL of this monomer-template solution was added dropwise to a deoxygenated mixture of hexane (21.5 mL), AOT (0.70 g) and Brij 30 (1.50 g), and stirred continuously for 30 min.

After the immunoprecipitation, the steps for washing, elution of chromatin, and DNA preparation were performed as described in the manual of the ChIP-IT Express kit (Active Motif). 2 µl of each of the resulting DNA solutions were used as template in standard PCR reactions and the PCR products were analyzed by Southern blotting.

The working solutions were diluted to 10 ng/μl as PCR templates.

The working solutions were adjusted to10 ng/ μl as PCR templates.

For the standard reaction, samples were diluted with a known volume of TE to give an expected final DNA concentration in the range 1 5 ng/µl, and 7.5 µl of this template DNA solution was mixed with 7.5 µl PCR mastermix to give final concentrations: 1× HotStar PCR buffer (Qiagen Ltd ,Crawley, UK), 1.5 mM MgCl2, 200 µM each dNTP, 1 µM of each primer.