Sentence examples for template solution was from inspiring English sources

The template solution was denatured by the addition of 3.5 µl buffer DLB for 5 minutes at room temperature.

For simultaneous detection of HBV and HCV, 2.5 μl of each template solution was added.

Each template solution was split into two equal parts with half serving as an original template for the measurement of the damaged/relaxed mtDNA fraction and the other half heat-treated (95°C for 6 min on a PCR machine) to quantify total amount of mtDNA [ 31].

Subsequently, 1.0 mL of this monomer-template solution was added dropwise to a deoxygenated mixture of hexane (21.5 mL), AOT (0.70 g) and Brij 30 (1.50 g), and stirred continuously for 30 min.

The corresponding data of the three template solutions were analyzed by PCA, obtaining 100% recognition.

The organic template solutions were made up to a concentration of 0.10% m/m.

Master mix/primer plus primer probe solutions and template solutions were separately loaded onto 96 well source plates.

For the standard reaction, samples were diluted with a known volume of TE to give an expected final DNA concentration in the range 1 5 ng/µl, and 7.5 µl of this template DNA solution was mixed with 7.5 µl PCR mastermix to give final concentrations: 1× HotStar PCR buffer (Qiagen Ltd ,Crawley, UK), 1.5 mM MgCl2, 200 µM each dNTP, 1 µM of each primer.

A rapid and valuable tool to evaluate the interaction between the functional monomer and the template in solution was the spectroscopic method.

This template and primer solution was mixed with the above reaction mix (reaction buffer, nucleotides and DNA polymerase) and incubated protected from light at room temperature for 1 2 hours.

The DNA in the denaturation solution was separated, templates were washed with washing buffer, transferred to a PSQ 96 SNP plate, and annealed with a sequencing primer (5′-CCACTCCATCGAGATTT-3′) in buffer at room temperature.