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For the collection of the reference set, we randomly select half of the samples from each motion set as the template samples, and the remaining samples are used for test and analysis.
Furthermore, "no reverse transcription" and "no template" samples were included as negative controls for each reaction.
What are the true limits of DNA interpretation for mixtures and low template samples?
The target gene names, RefSeq numbers and primer sequences are shown in Table 4. Triplicate cDNA template samples were amplified and analyzed in the Prism 7000 sequence detection system (Applied Biosystems).
Triplicate cDNA template samples were amplified and analyzed.
Wild type and no template samples were added in each assay as positive and negative controls.
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A template sample manual molding was used.
DNA contamination within the RNA template sample was checked either by use of no RT controls or using primers spanning introns.
Each PCR run included "no template" sample and all tests were performed in triplicate.
The 200 ng gDNA template sample was amplified separately after evaluation of the genotyping performance of the wgaDNA produced from 1 – 100 ng gDNA.
However, required prerequisites for the use of 454 sequencing for repeat quantification and reconstruction are that the template sampling is random and that the sequencing does not introduce a bias towards certain sequences.
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