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qRT-PCR reactions using the cDNA as a template revealed a shift in the amplification curve between reactions using either the native luc or synthetic luc* primer pairs.
In the related species V. pannonica, initial investigation of the VicTR-A repeats, using probe amplified in a PCR reaction with genomic DNA as a template, revealed a different hybridization pattern for their eccDNA, consisting of the smear with spots spaced by about 180 bp which could not be derived from multiples of the monomer length (Fig. 3J).
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Homology modelling of soft (RR-1) cuticular proteins using bovine plasma retinol binding protein (RBP) as a template revealed an antiparallel β-sheet half-barrel structure as the basic folding motif (Hamodrakas et al., Insect Biochem. Molec. Biol. 32 (2002 15777).
At 95 SNPs, comparison of TaqMan genotypes generated from MDA product and gDNA templates revealed a very good concordance rate but a higher failure rate for MDA product compared to gDNA.
RT-PCR assays using extracted RNA from normal and scrapie-infected hamsters as the templates revealed a 187 bp product with Tau-2-specific primers and a 127 bp fragment with Tau-4-specific primers, possibly representing the tau isoform of 372 amino acid residues and isoform of 432 amino acid residues, while the primers for Tau-6 failed to produce positive product (data not shown).
Indeed, THOC5/FMIP specific PCR analyses using genomic DNA as a template revealed that exons IV/V were deleted from these organs, including spleen after only one × times poly (I C) injection.
Amplification with cyt b specific primers using genomic DNA as template revealed the presence of an intron of about 1500 bp length after the codon for glycine at amino acid position 143.
A scatterplot of QFI-PCR and Qubit DNA template functionality revealed a linear trend for templates with at least 3% QFI by qPCR (R = 0.66; N = 86).
Thin films of this material were synthesized by block copolymer template-directed, evaporation-induced self-assembly, resulting in a periodic orthorhombic structure with Fmmm symmetry that, after template removal, revealed a pore size of around 7.5 nm.
Full-genome sequencing of the cloned BAC template, pBeloR26, revealed a number of differences throughout the genome when compared to the full-length consensus sequence of the cDNA used for the cloning procedure (see Table 1).
In combination with genomic mapping of the ST40 draft genomes against the D32 template, results revealed a comparably high level of diversity in plasmid content [ 81] and strain-specific phage patterns.
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