Sentence examples for template required for from inspiring English sources

Based on our model built from in vitro experiments, phosphorylation of N-Spc110 by Cdk1 (S91, S91) and Mps1 in S phase promotes the binding and oligomerization of γ-TuSC to form the template required for MT nucleation.

To examine the minimal amount of DNA template required for successful amplification using S-DIP and STR primers as well as L-DIP and STR primers, we amplified serial dilutions of DNA template from individuals heterozygous for the DIP allele, regardless the STR genotype.

Without the repair DNA template required for HDR, the majority of mutations caused by Cas9 are expected to be INDELs, SNPs or MNPs that initiate within the protospacer sequence, which is the 20 nucleotide (nt) sequence 5′ of the PAM (Fu et al., 2013).

In vitro, two components are minimally required for human telomerase activity; telomerase reverse transcriptase (hTERT), the protein catalytic and often rate-limiting subunit of telomerase, and the telomerase RNA (hTR), an RNA template required for the synthesis of de novo telomeric DNA [ 9].

A novel template selection strategy was employed to reduce the number of templates required for matching by up to 50%, while providing comparable performance with known approaches.

Hence, in experiments using 1000 cells, the least number of templates required for further analysis was 121 copies per cell.

Therefore, as an alternative strategy, viruses stained with SYBR Green (Invitrogen) were sorted directly onto cooled agarose beads applied to 'multi-well' polytetrafluoroethylene (PTFE) microscope slides to increase virus capture efficiency, as well as, to maximize the recovery of high-quality template DNA required for the MDA reaction.

Therefore, an ALL-specific template was required for successful MRD detection.

The amount of template gDNA required for the MDA reaction is also a critical determinant of genotyping performance because it is generally unacceptable for forensic purposes.

Secondly, two separate IDLVs are necessary to provide the two ZFNs and a third IDLV needs to be included if a donor template is required for homologous recombination.

The enzyme pauses after each nucleotide addition and rarely translocates on the DNA template as required for multiple rounds of synthesis.