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Next, we plotted the actual extent of the full length products in the 50-nt (Fig. 1D) and 137-nt (Fig. 1E) template reactions, which corresponds to the total IAV Pol activity in each reaction.
Negative (without template) reactions were performed within each assay.
The plasmid template reactions were also run in triplicate.
NT and non-RT RNA template reactions were used as negative controls.
Following template reactions, templated libraries were assessed for quality using the Ion Sphere Quality Control Kit (Catalog # 4468656) and Qubit fluorometer (Life Technologies).
These pathways were developed into template reactions to which each of iJL1678-ME's pORFs could be applied to (Additional file 1).
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Ratio of outer and inner primers, amount of DNA template, reaction temperature and time were optimized.
Propyl substituted 4-arylimino-1,2,3-trihydroacridylnickel 4-arylimino-1,2,3-trihydroacridylnickel 4-arylimino-1,2,3-trihydroacridylnickel 4-arylimino-1,2,3-trihydroacridylnickel
Complexes 1 and 2 ware prepared via a metal-assisted template reaction from the unsymmetrical pyridyl ketone precursor 2- OCMe -6- 2,6- iPr2-C6H3NCMe -C5H3N.
The cDNA template reaction contained Assay On Demand Gene Expression primers (see below; Applied Biosystems, Foster City, CA) and TaqMan® Fast Universal PCR no AmpErase UNG master mix (Applied Biosystems, Foster City, CA).
A negative control (no template) reaction was also performed for each primer pair.
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