Sentence examples for template reaction from inspiring English sources

Ratio of outer and inner primers, amount of DNA template, reaction temperature and time were optimized.

Propyl substituted 4-arylimino-1,2,3-trihydroacridylnickel 4-arylimino-1,2,3-trihydroacridylnickel 4-arylimino-1,2,3-trihydroacridylnickel 4-arylimino-1,2,3-trihydroacridylnickel

Complexes 1 and 2 ware prepared via a metal-assisted template reaction from the unsymmetrical pyridyl ketone precursor 2- OCMe -6- 2,6- iPr2-C6H3NCMe -C5H3N.

The cDNA template reaction contained Assay On Demand Gene Expression primers (see below; Applied Biosystems, Foster City, CA) and TaqMan® Fast Universal PCR no AmpErase UNG master mix (Applied Biosystems, Foster City, CA).

A negative control (no template) reaction was also performed for each primer pair.

Every step was performed along with a sample of genomic DNA from human tissue as a positive control and a no template reaction as a negative control, respectively.

Next, we plotted the actual extent of the full length products in the 50-nt (Fig. 1D) and 137-nt (Fig. 1E) template reactions, which corresponds to the total IAV Pol activity in each reaction.

Negative (without template) reactions were performed within each assay.

The plasmid template reactions were also run in triplicate.

NT and non-RT RNA template reactions were used as negative controls.

Following template reactions, templated libraries were assessed for quality using the Ion Sphere Quality Control Kit (Catalog # 4468656) and Qubit fluorometer (Life Technologies).