Sentence examples for template range from inspiring English sources

Using AMV-RT, Chandler et al. [12] assessed inhibition of qPCR at low template concentrations (2 fg – 2 pg), while Levesque-Sergerie et al. [10] tested detection limits of five commercial reverse transcriptases on a template range of 10 2,000 ng.

For this, each cDNA sample was pooled and then used as the PCR template (range of 50, 25, 12.5, 7.5, and 3.75 ng).

Furthermore, outside the optimal template range, several data points displayed values that were outside the feasible range of the assay (< 0% or > 100% methylation) and therefore were removed from the analysis (Table  1 and Figure  1A,B).

Consistent with a previous study [ 23], the data demonstrate how the variability of measurement can increase when the REs are used within a range of template % methylation that is not optimal for accurate PCR quantification, e.g. standard deviation measurements are generally higher in the 0-25% methylated template range with the MDRE digestion (Table  1, Figure  1A,C).

The MCC spreads for different templates were of little significance: variation between the best and the worst performing template ranged from 0.1 for actives/true inactives experiments to less than 0.05 for the other two VS scenarios.

Amplification efficiency for each gene was calculated based on four dilutions of template ranging from 500 ng to 0.5 ng and the equation E = 10−1/slope-1, with slopes in the range slope = −3.3±0.1 and E = 2. mRNA abundances for each candidate gene were calculated as: relative transcript abundance = 2(−ΔΔCt).

We carried out this experiment for five similarity measures including EHD, CC, MI, SSIM, and ENT with different sizes of template ranging from w = 25 to w = 75 and for each size, ten times.

As shown in Table S-4 of the Supporting Information, false-positive miR-34a reported by the miR-34a assay using miR-34c as the template ranged between 9.81 × 10 9 and 1.15 × 10 8 ng across all data points of the miR-34c standard curve.

Although the microstructure of these films is mostly microcrystalline, minority carrier lifetimes for films on Si (100) range from 5.7 to 14.8 μs while those for films on SNSPE templates range from 5.9 to 19.3 μs.

Templates range from wedding photo albums, to music lists for the DJ or Band to seating charts.

The global sequence identities between targets and their templates range from 13 to 33%, and the local sequence identities of the SSE in the ROI range from 0 to 56%, see Table 1.