Sentence examples for template quantities from inspiring English sources

High-throughput cloning of cDNAs requires relatively high template quantities because rare transcripts should be amplifiable.

Amplicon template quantities were measured using a threshold cycle (Ct) method [ 47, 48].

In all cases, actin amplifications were more robust for tissue templates, reflecting higher template quantities in the parallel control reactions.

The qPCR protocol was optimized with respect to primer and probe concentrations, DNA template quantities, buffer composition, number of cycles, and cycle parameters (data not shown).

Until recently, one of the major bottlenecks of shotgun metagenomics has been the requirement for large initial DNA template quantities during library preparation.

For example, multiple-displacement amplification (MDA) uses phi29 DNA polymerase to produce millions of copies of template DNA and has been previously used to increase template quantities in samples prepared for metagenomic sequencing [ 11].

The fluorescence intensity produced is in direct proportion to the template quantity.

Primers and a dual-labeled probe were designed and used to identify and quantify DNA from the target species in blind tests of unknown samples alongside a standard template quantity series.

Threshold cycle (Ct) was used to calculate the relative template quantity as the manufacturer's recommendation using β-actin as an internal control.

We did not observe a clear relationship between input template DNA concentration and 454 sequence read yield or quality; evaluation of the total number of reads relative to the number expected versus input template quantity did not show a template dependent difference in the read ratio.

The template quantity was regulated to be uniform using the ACTIN gene [ 54].