The requirement of the Fluidigm Access Array system for high template amounts and its sensitivity to variations in template quality rendered it unsuitable for bisulfite PCR applications utilizing FFPE DNA.
Additionally, the cases and controls used in the study by Field et al. were extracted using different techniques potentially introducing variation in template quality as a contributor to the poor performance of real-time QPCR.
The tumor cell population within the tissue under evaluation must be isolated, the template quality, operator variability, subjectivity in data analysis and reporting are technical aspects that must be considered [ 14, 15].
From these libraries, oligonucleotides representing 5257 putative transcripts as well as oligonucleotides representing unique sequences to control for nonspecific hybridization and template quality were designed and spotted, resulting in the 6K skeletal muscle-specific oligonucleotide microarray.
The number of amplification fragments in each primer combination is decided by the template quality, primer sequence, PCR reaction conditions, competition between potential amplicons, and base mismatching between primer and template (Smith and Williams 1994; Bussell et al. 2005).
Zhang, Y. & Skolnick, J. Scoring function for automated assessment of protein structure template quality.
15 It is often said that 'chronic diseases require a complex response', 10 and that structured care, for example, by using checklists or templates, is a mark of quality in chronic disease management.
Samples that yielded more than two haplotypes (probably due to PCR jumping [ Pääbo et al., 1990]) and samples which failed in a 12.2-kb PCR amplification as a consequence of poor template DNA quality, were amplified in two overlapping 7.4-kb and 8.1-kb fragments spanning exon 5 to intron 13 and intron 9 to exon 17, respectively.
Findings from this study can be employed as a template for quality control either in raw materials, extracts or finish products of O. stamineus leaves extracts and would be an important tool for selection of potential extracts for further product development study of O. stamineus leaves extracts.
