Sentence examples for template quality from inspiring English sources

The application of polymerase chain reaction (PCR) to detect microorganisms is largely affected by DNA template quality prepared from food samples.

Its success mainly depends on the template quality in the ROI and its surrounding.

Universal 28S ribosomal DNA primers D3A and D3B [76] produced a band at 400 bp, confirming DNA template quality.

All DNA extracts produced a band at approximately 400 bp using 28S arthropod primers, confirming DNA template quality (Figure 1A, bottom).

QFI-PCR respects that high template quality is not the sole sample-level variable that drives successful library preparation.

PCR using universal primers targeting bacterial 16S rRNA conserved sequences was also performed to ensure template quality.

What we have done is to create a template of quality, available as a guide.

Using this template, the quality of the selected studies was independently assessed by the two senior authors (B.R.W. and P.J.B .. Disagreement concerning study quality was moderated by consensus.

First, it depends on template DNA quality.

Total RNA extraction and reverse transcriptase (RT) reactions were performed after DNAse treatment and template RNA quality, including degradation and DNA contamination, was controlled as described previously [49] [51].

In our PCR reaction, primers specific for the BRAF gene allow the amplification of a non-polymorphic gene area, which was used as an internal control to check for template DNA quality as well as potential PCR inhibition.