The MIP with 4-chlorophenyl 4-vinyl phenyll carbonate as template was proved to be the best one, with ethylene glycol dimethacrylate (EGDMA) as cross-linker, 2,2-azobisisobutyronitrile(AIBN), and chloroform as initiator and porogen, respectively.
If available, the amplification was repeated with an alternative template which proved to be efficient for ≥ 78 % of these ORFs (Table 2).
Although it is necessary to optimise the dsDNA Fragmentase reactions for a given template, this proved to be an efficient method for fragmenting the long-range PCR products of a large number of individuals, and produced consistent results.
Because the fosmid cloning technique for generating template DNA proved to be rapid, efficient and cost effective, it was also chosen to sequence the cpDNA of H. akashiwo NIES293, West Pacific strain.
In recent years, monolayer colloidal crystal template (MCCT) has proved to be an effective approach for fabricating ordered nanoarrays due to their low cost, flexibility, and various morphologies [22, 23].
The kinetic template effect has been proved to be a useful tool to improve the yield and selectivity in the synthesis of macrodiolide 3.
Among various synthetic processes, template synthesis has been proved to be a versatile and simple approach for the preparation of many nanostructures, such as conductive polymers, metals, semiconductors, carbon and other materials [7 10].
This bis-template and bis-reductant method is proved to be an effective way to prepare nanowires with special structure.
A series of libraries were designed using the 1- cyclopropylmethyl -2-alkyl-4,5,6,7-tetrahydro-1H-imidazo[4,5-c]pyridin-5-ium templates 2a–b, and Sulfonamide derivatives 1- cyclopropylmethyl -2-alkyl-4,5,6,7-tetrahydro-1H-imidazo[4,5-c]pyridin-5-ium
