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Exact(4)

In addition, the template products were validated by DNA sequencing performed with the Applied Biosystems DNA Sequencer (3130xL) using the BigDye® Terminator chemistry and showed the highest similarity to zebrafish NUCB2A in a BLAST search.

If genomic DNA had been the template, products greater than 18 kb would have been obtained.

After PCR amplification using sunflower genomic DNA as template, products with the expected sequence were obtained for 10/19 loci.

Melting curve analysis and agarose gel electrophoresis were carried out to assess the size of the template products.

Similar(55)

The ontology is used to represent and to infer knowledge about templates, products and their relations.

When PatternEQ releases its templates product, we'll check back in with the company to see how quickly it is growing.

In contrast to primer-dependent RdRPs that have a spacious front channel to accommodate the template-product RNA duplex, the flavivirus RdRP has a priming element as a thumb domain insertion, partially occupying the front channel to facilitate the de novo initiation process.

Shotgun template extension products were purified using the CleanSeq dye-terminator removal kit (Agencourt) and amplicon extension products were purified using DyeEx 96 well plates (Qiagen) according to the manufacturer's protocols.

The discrepancy might be attributed to the method used for quantitation of the template and products (PicoGreen DNA quantitation vs. NanoDrop spectrophotometry).

In addition, when DNA of the three different BoHV-1 subtypes was used as template, amplification products could not be digested by BstEII (data not shown).

*Yield and fold amplification may be overestimated for low starting concentration due to non-specific or template independent products (see text).

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