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Furthermore, the high copy number and structural stability of the pUC19-based pMG vectors in Escherichia coli facilitates template production for target host transformation.
And Jackson said Sendicate is also launching a concierge/managed version, which will include "dedicated account management, design and strategic services, template production, results optimization, custom integrations, etc".
In the template production process, the process parameters of template projection were oxidation voltage and electrolyte concentration.
About 100 ng of double-stranded cDNA was subjected to standard AFLP template production.
Alternatively, the efficiency of mutation analysis could be increased by modifications in DNA template production from large populations.
DNA was prepared using alkaline lysis [ 3] with the following modifications that have been implemented for the standard GSC template production pipeline.
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The T7 RNAP also appears to be less suitable for this application, because, while it transcribes partially single-stranded DNA templates, production of the hybrids with the proper ssDNA toeholds was not successful.
This popRSV - CDK4-R24C construct was used as the template for production of the CDK4 kinase dead mutant, made by altering aspartic acid 158 to asparagine using a point mutagenesis kit from Stratagene (Cedar Creek, TX) [42].
Although the mechanism of HCV replication is not completely understood, viral replication is assumed to involve the synthesis of a negative-strand RNA molecule (antigenomic HCV-RNA) that acts as a template for production of a positive-strand RNA molecule (genomic HCV-RNA) [2], [15], [20].
For example: DNA acts a template as production of RNA.
DNA acts a template as production of RNA.
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