In addition, the template products were validated by DNA sequencing performed with the Applied Biosystems DNA Sequencer (3130xL) using the BigDye® Terminator chemistry and showed the highest similarity to zebrafish NUCB2A in a BLAST search.
If genomic DNA had been the template, products greater than 18 kb would have been obtained.
After PCR amplification using sunflower genomic DNA as template, products with the expected sequence were obtained for 10/19 loci.
Melting curve analysis and agarose gel electrophoresis were carried out to assess the size of the template products.
When PatternEQ releases its templates product, we'll check back in with the company to see how quickly it is growing.
In contrast to primer-dependent RdRPs that have a spacious front channel to accommodate the template-product RNA duplex, the flavivirus RdRP has a priming element as a thumb domain insertion, partially occupying the front channel to facilitate the de novo initiation process.
The ontology is used to represent and to infer knowledge about templates, products and their relations.
Purified PCR products were directly sequenced using ABI BigDye sequencing mix (1.4 ml template PCR product, 0.4 ml BigDye, 2 ml manufacturer supplied buffer, 0.3 ml primer, and 6 ml H2O).
Purified PCR products were directly sequenced using ABI BigDye sequencing mix (1.4 µl template PCR product, 0.4 µl BigDye, 2 µl manufacturer supplied buffer, 0.3 µl primer, and 6 µl H2O).
In the primary reaction, the ligated DNA was used as a template, the product was used as the template in the secondary reaction, and the tertiary reaction was achieved in the same way.
By introducing phosphorus sacrificing template, the product delivered an approximate 2.7 folds higher specific capacitance with faster ionic and electronic transport than that of the counterpart synthesized without phosphorus participation.
