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A query template produced in this manner can be designated as the ' precise template'.
The template produced in Figure 5A was applied to three replicate samples each of unstimulated and influenza-stimulated cells.
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The PCR reactions were linear over a range of eight log10 of synthetic template, produced one peak in melting curve analysis and exhibited a good correlation between Cq and template concentration.
Control reactions in which wild type genomic DNA was used as a template produced the expected PCR product in all reactions; no PCR products could be detected using Δ nfsA-G genomic DNA or when mock cDNA samples in which either the reverse transcriptase or template RNA was omitted was used as template.
For example, a template produced for an experiment studying development in mice would include fields for mouse strain, sex, developmental stage, age and so on.
It can be seen from Figure 6 that the megakaryocyte template produced a greater quantity of the amplicon in each case, reflective of the availability of template rather than an increased proportion of AS message in these cells.
The resulting HARDI template was produced in ICBM-152 space based on Turboprop diffusion data, was shown to resolve complex neuronal micro-architecture in regions with intravoxel heterogeneity, and contained fiber orientation information consistent with known human brain anatomy.
The previously developed "IIT DT brain template" was produced in ICBM-152 space, based on a large number of subjects from a limited age-range, using data with minimal image artifacts, and non-linear registration.
The reproduced template is then compared with the keystroke template produced by the alleged intruders.
Use of cobalt-containing MOFs as a sacrificial template produces uniformly distributed Co3O4 nanoparticles in the carbonaceous matrix, alleviating the problems of using only Co3O4 as the cathode material.
These ORF clones were derived using the human MGC collection as template and were produced in two formats: with and without stop codons.
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