Sentence examples for template present in from inspiring English sources
This could cause differential PCR amplification of the mixed template present in the faecal samples [47].
The resulting products were purified and subjected to second stage individual specific PCR for each template present in the sample and analyzed on agarose gel.
We showed here that the mutated template present in the range of 25%to30%0% could be really difficult to detect during sequencing analysis.
The relative Ct (threshold cycle value) method was used to estimate the initial amount of template present in the reactions by Applied Biosystems SDS 2.0.
We also observed greater variance associated with sequence coverage among samples for the mitochondrial genome, which could reflect a difference in the amount of mitochondrial template present in each DNA sample (Additional file 3: Figure S4; Additional file 5).
It is well-known that nucleotide variations in the annealing positions of primers and probes affect the sensitivity and efficiency of this type of reaction, as they can lead to putative underestimations of the actual amount of template present in a sample.
The template presented in this paper has been used for this purpose by the authors and is promising.
Accordingly, the initial template was somewhat modified throughout the analysing process, resulting in a final template presented in the Results section.
The analyzing process was carried out by identifying higher-order themes and further scrutinizing the contents of these themes to identify and differentiate lower-order themes, resulting in a final template presented in the results section.
This could be due to the lower diversity of templates present in sorted compared to filtered samples, since the latter include both prokaryotes and eukaryotes and both autotrophic and heterotrophic populations [13].
Although Illumina sequencing is a highly efficient method for DEG screening, false positives still occur because of the sensitivity of this technology to templates present in DNA samples [ 23].
