Sentence examples for template preparation was from inspiring English sources

Single strand template preparation was performed as previously described [26].

Single strand template preparation was performed as previously described [23], and oligonucleotides complementary to barcodes included in the probes were used as sequencing primers (Table 3B).

Sequencing template preparation was made with the TempliPhi DNA sequencing Template Amplification Kit (Amersham Biosciences).

The amount of library required for template preparation was calculated using the template dilution factor calculation described in the protocol.

Template preparation was performed using the Ion OneTouch System with the Ion OneTouch 200 Template Kit v2 DL Life Technologiess).

Template preparation was performed using BluePippin (Sage Science) size-selection system including DNA damage and end repair steps and ligation to hairpin adapters.

A typical procedure of the template preparation is based on a two-step self-organized anodization of aluminum carried out at the temperature of about 1 3 °C.

The universal fragments used for template preparation were obtained by PCR on plasmid 1751 (Fig. S1A) with primers 511/2522, 511/9152 and 33/2511 for fragments from 5 to 7 respectively (Fig. S2).

Therefore, the inclusion of a preheating step in the template preparation is crucial for the accurate mtDNA measurement.

The acquisition of genotypes by sequencing is very accurate and rapid once template preparation is multiplexed using barcodes, informatics pipelines are established, and criteria for assigning genotypes have been validated.

The Illumina MiSeq (for library validation) and HiSeq 2000 sequencing systems (Illumina, CA, U.S). were used for sequencing, and the library preparation kits were purchased from New England Biolabs (MA, U.S .. Sequencing library construction and template preparation were performed according to the NEB library preparation protocols.