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The libraries were prepared according to the 20-kb Template Preparation Using BluePippin™ Size-Selection Sage Science Sage Science
For C. micaceus unamplified libraries were generated using the Pacific Biosciences standard template preparation protocol.
Template preparation was performed with the Ion OneTouch™ 2 System (OT2) using the Ion PI™ Hi-Q OT2 200 Kit.
A simple extraction procedure using RNAsecure™ was developed for template preparation.
Careful consideration of the assay design, template preparation, and analytical methods are essential for accurate gene quantification.
The template preparation method is based on photo-lithography, micro-contact printing and carbon nanotube chemical vapor deposition techniques.
Single strand template preparation was performed as previously described [26].
Next we sought a way of departing from cloning-based template preparation methods.
Genomic DNA was extracted using the High-Pure PCR Template Preparation Kit (Roche).
Cell Proliferation Reagent WST-1, and High Pure PCR Template Preparation kits were obtained from Roche Applied Science.
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Total genomic DNA was extracted using High Pure PCR Template preparation Kit Roche Cat. no.
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