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Given the shape template P and the observed edge image, the likelihood function is defined as.
The magnitude of the difference in the C t) between PBMC and the plasmid template correlated with the concentration of the template (P < 0.05), indicating that the PCR efficiencies were significantly different.
The number of amplifiable copies of the p14 and COL2A1 genomic regions detected by MSRE-dPCR, mock digestion conditions and MethyLight dPCR was less than the values based on Qubit analysis (p < 0.05) with the exception of the MDRE dPCR assay (0% methylated template (p = not significant)).
A single nucleotide is represented as the four-dimensional probability column-vector p that comprises the probability of that nucleotide being one of the four nucleotides A, C, G, or T. The action of Taq polymerase on template p is modelled as a linear Markov operator T that pairs adduct q = Tp against the template during synthesis.
The 2 × 2 bound oligonucleotide produced a greater difference in nuclease sensitivity between the wild-type and mutant template sequences (p < 0.001) and almost complete degradation of mutant template was achieved, however, the wild-type/2 × 2 was also sensitive to the nuclease (approximately 50% reduction relative to the control wild-type complementary template; p < 0.001).
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Surface molecular imprinting was further conducted by assembling 1-dodecanethiol, p-toluenethiol, and p-nitrophenol (p-NP) on lamellar ridge-Au surface followed by removing molecular template p-NP.
Noh, J.-H., Patala, R. & Meijboom, R. Catalytic evaluation of dendrimer and reverse microemulsion template Pd and Pt nanoparticles for the selective oxidation of styrene using TBHP.
The 5′-upstream 5′-upstream91 bp) of the human regiongene was prepared using human genomic DNA as a template (p-full).
No significant difference between the templates (P > 0.05) was found.
There was a 3-hour reduction in the time required to initiate EEG recording using the templates (P < 0.001).
However, the 2 × 1 oligonucleotide induced a significant difference in cleavage between wild-type and mutant templates (p < 0.05), while maintaining no significant difference between the wild-type/2 × 1 and the wild-type complementary bound templates (p > 0.05).
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