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In many of these applications, the portable device may be required to transmit an iris image or template over a narrow-bandwidth communication channel.
We designed a TLS assay which monitors primer elongation on a synthetic oligomer template over a single UV-induced lesion, either a cys syn cyclobutane pyrimidine dimer (CPD) or a pyrimidine (6-4) photoproducthotophotoproduct
A standardized template is centered over the fovea and used to divide the macula into these sectors [ 39]. Figure 10 shows this template over a retinal fundus image.
In contrast to polyadenylation, termination of transcription occurs where Pol II is released from the DNA template over a range of about 200 (yeast) to 1500 (humans) nt downstream of the PAS [48,49].
Melting curve analysis was performed to confirm the amplification specificity by heating the template over a 35°C temperature gradient at 0.5°C/sec from 60 to 95°C while continuously monitoring the fluorescence.
The efficiencies of target and housekeeping genes were determined by qRT-PCR on serial dilutions of RNA template over a 100-fold range [ 59], with similar results (data not shown).
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In cases where the devices require a query to a master database for identification, it may be required to transmit captured images or templates over a narrow-bandwidth communication channel.
Vapor deposition of perfluoroalkylsilane thin layers improved the thermal stability of the colloidal template over 100 °C, providing a route to preparation of hollow architectures with gold thin layers supported by PDMS nanostructures.
For template-based modeling, these advances allowed improvement in predicted structures relative to their initial template alignments over a wide range of target template homology.
A regression line between the mean Ct values and viral template concentrations over a 1 107 dilution range with an r2 value (0.9916) and a slope (−3.36) and the coefficient of variation (intra- or inter-assay is <2% and <4%, respectively) indicated that the assay was highly reproducible.
But the murder set a grim template over the following two centuries for a series of lone gunmen who attempted to further their own views by picking up a firearm and training it on their nation's ruler.
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