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Break-induced replication (BIR) has been invoked as an HR mechanism for the repair of one-sided DSBs that may arise when a replication fork collapses at a nick in the template or when telomeres erode [4], [5], [6], [7].
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CNFpred performs especially well when only distantly related templates are available or when the proteins under consideration have sparse sequence profiles.
Primers were designed using Primer3 software (frodo.wi.mit.edu/primer3/) and quail genomic sequence as a template or chicken sequence when the quail sequence was not available.
Groucho also did not stably associate when the factors were bound to naked DNA, or when templates were assembled with tail-less histone H3 and H4 nucleosomes, or when assembled with highly acetylated chromatin [ 40].
This can arise due to inherent bias in the amplification conditions (variable primer annealing, amplicon sequence content, suboptimal reaction temperature or salt concentration, etc)., or when initial template concentrations are insufficient to generate copy numbers exceeding the minimum detection threshold.
We excluded visits when a template was not used to document the encounter (i.e., the encounter was instead documented with a handwritten or dictated note) or when the template was of poor quality such that information could not be abstracted.
A increase of maximum key size is achieved in various scenarios, e.g., when applying several biometric templates at enrollment and authentication or when increasing the desired false rejection rates.
For example, if a gene is located on chromosome 1A, the PCR product will not be generated in the N1AT1D or N1AT1B template when 1A genome-specific primers are used.
Both assays were highly sensitive and detected the presence of 1 pg of donkey template DNA or 25 pg of horse template DNA when assessed using dilutions of DNA in water.
This may be due to a stochastic or inevitable loss of the template DNA when dealing with a single copy.
Changing the basic template now, when foreclosures are more common than groundbreakings, is a little late, some say.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com