Sentence examples for template of total from inspiring English sources

cDNA was prepared on a template of total RNA extracted from upper leaves taken from infected N. benthamiana and S. lycopersicum.

The fragment encoding the human Cx43 sequence was obtained by RT-PCR performed on a template of total RNA isolated from GliNS2 cells using the following primers: 5′-TATATACCGGTATGGGTGACTGGAGCGCCTT-3′ and 5′-CGGGATCCCGCTAGATCTCCAGGTCATCAG-3′.

PCR with specific primers on the template of total M. musculus DNA gave the ladder for TRPC-21A as well as for MaSat, indicating the characteristic feature of the satDNA, also caused by variable monomers organized in HOR (data not shown).

From the TG results, it can be summarized that the MCM-41 nanoporous silica synthesized from three subsequent cycles contained almost the same amount of template (total weight loss of 36 to 41 wt.% in the range of 120°C to 500°C), demonstrating that the consumption of the organic template during the formation of MCM-41 was almost constant in each step of the multi-cycle synthesis.

For synthesis of cDNA a template of 0.5 μg total RNA was used.

Here the number of template representatives was kept to be 20% of total templates.

Typically 500 ng of template total RNA and 250 ng of random hexamers were used per reaction.

For 3′ RLM-RACE, all the reverse transcriptase steps were the same as 5′ RLM-RACE, except for the template where 1 μg of total RNA was used.

For PCR and Q-PCR analysis 2 ul of template in 20 30 ul of total reaction were used.

ERK1 RNA was obtained by one step RT-PCR performed with a template on 100 ng of total RNA extract from Rat brain.

To remove any contaminating genomic DNA from the RNA template approximately 2 μg of total RNA was DNase I treated using the DNA-free system (Ambion).