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Using the template of the first PCR reaction, allele-specific primer pairs was used to test for the presence of the allelic variants from FC27 and IC of the families of the msp2 region.
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A 1-μl aliquot of the first-round PCR product was used as the template of the second-round PCR reaction.
Herein, we report the design, functional, and structural characterization of a potent and selective MC4R full agonist and present the studies of proteolytic stability and in vivo properties of this template for the first time.
The double-stranded cDNA was precipitated with ethanol, air-dried and used as template for the first round of in vitro transcription of cRNA with MEGAscript enzyme mix (Ambion) at 37°C for 7 h.
Their lineup – male diva Marc Almond, keyboard wiz Dave Ball – set the template for the first half of the 80s.
Hull's modest motel-casino became the template for the first generation of resorts on the Strip.
The purified double stranded cDNA served as template for the first round of aRNA transcription.
The purified double stranded cDNA served as the template for the first round of in vitro aRNA transcription.
A PCR product containing the lpoA Δ58-252) allele was then generated via overlap extension ('stitching') PCR (Heckman and Pease, 2007), using PCR primers ANG235 x ANG239 and ANG241 x ANG242 with E. coli genomic DNA as template for the first round of PCR and ANG235 x ANG242 with first-round-PCR-products as template for the second round of PCR.
In the present study, we introduce a novel method for electrodeposition of long gold nanotubes within the pores of PC templates for the first time.
If you did not line up two pieces of felt together, then pin the template to the second piece of felt and cut it out as well.
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CEO of Professional Science Editing for Scientists @ prosciediting.com