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Regions of interest (ROIs) were drawn manually on a T2-weighted MRI template of rat brain around the striatum, brainstem, cortex, hippocampus, hypothalamus, thalamus, olfactory bulbs, and cerebellum around the whole brain and around the pituitary gland.
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Predefined volume of interest (VOI) analysis was performed based on a MRI-based functional template of the rat brain in Paxinos standard space, as described by Casteels et al. [10].
Templates for transcription of rat MT-1 3′UTR nucleotides 1 111 and the various mutants were generated by PCR from pc MTfull, or the appropriate mutant construct, with the following primers;- T7 Forward 5′- TAATACGACTCACTATAGGAGTGACGAACAGTGCTGCTG-3′ containing the promoter sequence (underlined) and Reverse 5′-CACATGCTCGGTAGAAAACGG-3′.
The summed images (all timeframes) of the reconstructed data were spatially normalized to an in-house created [11C]raclopride template of the Wistar rat brain.
The crystal structure of TxIA(A10L) bound to Ac-AChBP was used as a template to build homology models of rat α7, α3β2 and α4β2 nAChRs following the method described by Dutertre et al (2005).
The L-Snord115 expression constructs were generated via polymerase chain reaction (PCR) amplification of rat genomic templates.
The automatic naming process is based on the following three steps: 1) where possible, the human gene symbol is used as a template; 2) if this is not possible, a template is sought first in RGD and then in other sources of rat gene symbols; and 3) if neither of the previous steps proves fruitful the mouse gene symbol is used as a template.
Images from each data set were normalized to the mean image of the corresponding template rat using the affine transformation of AIR.
Briefly, modeling of human REP-1 was based on the 2.2 Å crystal structure of rat Rep-1 protein (PDB file: 1vg0) as the structural template (Abola et al. 1987).
The genomic organization of rat urocortin was determined using both cDNA and liver genomic DNA as templates for polymerase chain reactions.
Open image in new window Figure 4 Homology modelling of rat α3 and rat α6 bound with α-conotoxin LvIA using Ac-AChBP/LvIA as the template.
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